Product Name :
Phospho-Raf1 (S43) Recombinant Rabbit Monoclonal Antibody [SD087-05]
Predicted band size :
Observed band size :
Synonyms:
c Raf antibody C-raf antibody C-Raf proto-oncogene, serine/threonine kinase antibody CMD1NN antibody Craf 1 transforming gene antibody cRaf antibody Craf1 transforming gene antibody EC 2.7.11.1 antibody kinase Raf1 antibody Murine sarcoma 3611 oncogene 1 antibody 展开
Function :
Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk)and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-1 is activated in response to activation of a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. There is accumulating evidence that Ras p21 may play a role in activation of Raf-1 and may play the role of the messenger from membrane tyrosine kinases to Raf-1.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Ser43 of Human Raf1 aa 20-69 / 648.
Species reactivity:
Human, Mouse, Rat
Verify application:
WB
Coupling :
unconjugated
Clone number:
SD087-05
RRID:
AB_3070151
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.1329035-82-6 web Aliquot store at -20℃ or -80℃.3-Bromo-5-methoxyphenol In stock Avoid repeated freeze / thaw cycles.PMID:33630475
Storage buffer solution:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
73 kDa
Positive control :
NIH/3T3 cell lysate, 293T cell lysate.