Luorescence (ex = 295 nm; em = 340 nm) was followed working with a FP-8300 Jasco

Luorescence (ex = 295 nm; em = 340 nm) was followed using a FP-8300 Jasco fluorescence spectrophotometer. Enzyme Assays on Purified Enzymes. Initial velocity was measured spectrophotometrically at 486 nm…