Ation immediately after neurotrauma.proper conjugated secondary antibodies (Alexa Fluor 555 or 488, Cy

Ation right after neurotrauma.proper conjugated secondary antibodies (Alexa Fluor 555 or 488, Cy3, Molecular ProbesInvitrogen). Nuclei had been counterstained with 4,6diamidino2pheylindole (DAPI, 1:1000, SigmaAldrich). Specificity on the staining was verified…